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KMID : 1024420130170030271
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Food Engineering Progress
2013 Volume.17 No. 3 p.271 ~ p.275
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Development of Rapid Detection Kit for Aflatoxin B1 in Cereals
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Choi Sun-Mi
Kim Gi-Young
Lee Sang-Dae
Moon Ji-Hye
Om Ae-Son
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Abstract
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The aim of this study is to develop the one dot rapid detection kit that meets the limit of detection (LOD) of KFDA (10 μg/kg) for Aflatoxin B1 (AFB1). The fabrication process of the one dot rapid detection kit, such as gold nanoparticle and AFB1-BSA conjugation, and AFB1-polyclonal antibody concentration was optimized. Fabricated rapid detection kits were tested with the standard AFB1 solutions and the solutions extracted from contaminated maize samples. The LOD in the standard AFB1 solution and extracted AFB1 solution was 5 μg/kg for both. This result is lower than the LOD of KFDA in food (10 μg/kg) and satisfies the LOD of EU (2-12 μg/kg) and Japan (10 μg/kg) in food.
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KEYWORD
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Aflatoxin B1, Rapid detection kit, Cereals, Corn
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